Advanced Solutions for Organoid and Tissue Engineering

The material is designed to be stable during cell culturing and is provided as a coating in standard cell plates and intended for cells or flat tissue applications. The user can degrade the coating by simply adding a TAG enzyme at any point for collection or passage of the cells or formed tissue. 

• The Hydrogel coating is fully defined Xeno-free tunable to maintain the cell characteristics.
• Upon degradation of the cell culture carrier, any surface proteins, cell-cell, and cell-matrix contacts, as well as the extracellular matrix of the formed tissue, remain chemically and physically untouched. This significantly increases the survival rate of the cells and tissue to be father manipulated or implanted. 
• The hydrogel coating is degraded to small, water-soluble molecules and can be removed by simple washing, providing a free-floating, ex vivo produced tissue without any artificial scaffold. 

Sample Applications: 

Corneal Lamellae transplantation (Descemet membrane endothelial keratoplasty DMEK)

 Bioengineered Retina transplantation 

The material is designed to be cell responsive, allowing cell expansion and remodeling in three dimensions, and is intended for cells, organoids, and tissue applications. The user can degrade the scaffold by simply adding a TAG enzyme at any point for collection or passage of the cells, organoids, or formed tissue.  

• The Hydrogel composition is customizable for specific applications to direct the cell fate in the required linkage.
• It can be used for the transportation and transferring of cells, organoids, or tissues. 
• Upon degradation of the cell culture carrier, any cell-cell and cell-matrix contacts and the extracellular matrix of the formed tissue remains chemically and physically untouched.
• The hydrogel coating is degraded to small, water-soluble molecules and can be removed by simple washing, providing a free-floating, ex vivo produced tissue without any artificial scaffold. 

Sample Applications: 

Capillary network (Angiogenesis) 

Mammary gland organoid (mammary epithelial morphogenesis)

 For many diseases, tissue and organ transplantation provides the first-line and frequently the only life-saving therapeutic option. However, less than 15% of the global need for tissues and organs is met. For a number of these diseases, only a small portion of the organ needs to be regenerated to restore its function. Over the last decade, cell therapies as regenerative medicine were considered to be the most promising solution to overcome the donor shortage. Nevertheless, despite more than 4000 clinical studies, single cells therapies remain ineffective overall. Cell therapies of "solid tissues" are facing three main issues:

1. Maintaining cell morphogenesis.  
2. Cell viability during and after implantation
3. Cell migration away from the target area. 

At TissueGUARD, we developed a few solutions which can overcome the above issues. These technologies increase cell viability and prevent their migration from the injected area, thus enabling the therapeutic outcome. 

Contact us for more details. 

Sample Applications: 

Neovascularization (Ischemic diseases)